Subsequent CHARACTERISATION Out of Several ARTEMIA Populations Of North GREECE: BIOMETRY, HATCHING Services, CALORIC Posts And Greasy Acid Pages
Embolon, Thessaloniki, have been characterized about its setting of breeding and ploidy
BOWEN, S.T., DURKIN, J.P., STERLING, Grams. and you may CLARK, L.S. (1978) Artemia hemoglobins: genetic version within the parthenogenetic and you will zygogenetic populations. Bull., 155, 273287.
Cysts off parthenogenetic Artemia stresses amassed about Citros and you may Yards. Embolon saltworks within the Northern Greece are examined due to their prospective play with in aquaculture. The next characterizations was in fact performed: cyst and you may naupliar biometrics, tumor hatching services, greasy acidic reputation of nauplii, caloric posts out of nauplii kept during the twenty-five° C plus in a refrigerator (cuatro 8° C). The aforementioned investigations suggests that the two Artemia stresses studied showcase an excellent qualities for use from inside the aquaculture, particularly in culturing new-water species. New biometrical data regarding cysts, nauplii and you can grownups suggests a premier amount of similarity along with other parthenogenetic strains off individuals geographical sources, but specifically with tetraploid Artemia of Spain. Brand new Greek Artemia stresses cannot be considered as ‘sources to own aquacultural uses except if best management of the fresh saltworks is assured.
Once the brine shrimp Artemia try off highest monetary pros, it is vital to evaluate the newest ‘commercial characteristics of numerous populations for their possibility application in aquaculture. Within the previous degree, two Northern Greek Artemia populations found in Citros, Piera and you can Yards. These were found to be parthenogenetic and you may tetraploid (Abatzopoulos mais aussi al., 1986). Investigation regarding degree on DNA reassociation Kinetics as well as on 13 enzymatic solutions of these two populations signify they get into you to species (Abatzopoulos ainsi que al., 1987).
Biol
Within report i breakdown of the fresh new biometrical qualities from cysts, nauplii and adults, into hatching properties away from cysts, i.elizabeth. hatching price, hatching percentage, hatching results and you may hatching production, to your changes in deceased weight and caloric content from instar-I nauplii was able at room-temperature and you may cold storage criteria, as well as on new oily acidic users out of instar-We nauplii.
The 2 communities away from Northern Greek Artemia analyzed come from the saltworks out of Citros, Pieria (C strain), and you can on the saltworks out-of Yards. Embolon, Thessaloniki (Myself strain). Cysts was indeed collected during the 1983, canned according to Sorgeloos ainsi que al. (1978), and you can stored in closed plastic handbags from the -25° C, to own coming have fun with.
Mature brine shrimps were anesthezied for the chlorofrom-over loaded and you will measured lower than a good dissection microscope playing with good Leitz micrometer, The following specifications was basically performed : complete duration, abdominal length, maximal depth away from brood wallet, width out-of third abdomianal phase, width out of lead, duration of very first antenna, maximal diameter away from advanced vision, and length ranging from complex sight (Amat, 1980). Proportions was indeed did into the pet – decide to try dimensions = 50 dogs-from crazy cysts and you will cultured below practical labouratory conditions (fungus just like the food, heat off twenty-five + 1° C, and you can seawater out of 35% salinity). The data had been mathematically addressed of the research from covariance (Sokal Rohlf, 1981).
Hatching analyses was in fact achieved based on Vanhaecke Sorgeloos (1983). Hatching efficiency, hatching fee, hatching price and hatching returns had been analysed following the speed and you may hatching production was indeed analysed after the Sorgeloos ainsi que al. (1978), Bruggeman mais aussi al. (1980), Vanhaecke Sorgeloos (1982), and you can Vanhaecke Sorgeloos (1983), respectively.
Brand new hatching of your own cysts and the break up and you can type of brand new nauplii (instar-l in order to instar-III) were carried out with respect to the process demonstrated during the Vanhaecke e al. (1983).
The newest types of nauplii was dehydrated during the a keen incubator on 55°C having forty-eight several hours. Each naupliar stage analyzed, 4 to 6 duplicated examples, of about ten milligrams dead question was basically burned entirely from inside the absolute clean air as well as their caloric posts was counted within the a good Phillipson microbomb calorimeter.
Before studies, products have been homogenized into the an enthusiastic ultrasonic homogenizer (Sonifier B12) and you can total lipids had been extracted with regards to the kind of Bilgh Dyer (1959). Saponification and you may methylation have been did while the discussed because of the Shauer Simpson (1978). Oily acid methylesters was inserted for the a beneficial capillary column (twenty-five yards bonded silica; internal diameter; 0.32 mm; liquide stage : SILAR 10C, film occurrence: 0.3um) strung in the an effective Carlo Erba Mega 2350 gasoline chromatograph.